Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. There are two options here: Buy from Accuri, or make your own citric acid solution. It is cheaper and easier to make your own.

Setup Procedure for D Accuri™ 6 This part of the guide applies to BD digital flow cytometers using D Accuri™ 6 software. For the D Accuri™ 6, use the PE channel (FL2) for the reporter and the APC channel (FL4) for beads classification. The Setup Beads 3: Raw Beads are used to setup the machine. Setup Beads 1: FITC Beads and 2: PE Beads

BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. If you have questions or require further clarification, see the BD Accuri C6 Flow Cytometer Instrument Manual or contact Accuri Technical Support. 8. Place the mat in position (Figure 10). WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. 9.

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Tabbed views for collection, analysis, and statistics b. 3. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. (In this example, the file is FCS Express Import Demo.c6.) NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. 1.2 CFlow Plus v1.0.227.04 Software Capabilities BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. BD Accuri C6 Software provides the following features: Tabbed views for collection, analysis, and statistics routine. For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual.

Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. • Calibrationshould be performedin the same tube type as the experimental sample. It is small enough to easily fit on a benchtop, and can be placed in a laminar flow hood.

BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30

Procedure: SIP Clean, Starting up the system, Shutting down the  instructions (BD Accuri C6 Software User Guide). Finally, the Plant DNA. Analysis Template was opened. This template consists of the following plots and. BD Accuri™ C6 Plus Flow Cytometer는 쉬운 사용방법 및 간편한 유지 보수로 누구나 쉽게 사용 가능하며.

instructions (BD Accuri C6 Software User Guide). Finally, the Plant DNA. Analysis Template was opened. This template consists of the following plots and.

Senare dissocierades NPC till enstaka celler i N2B27-mediet plus 10 ng / ml bFGF färgades med Annexin V och propidiumjodid (PI) enligt tillverkarens manual. Sorter eller BD Accuri C6 flödescytometer (BD Accuri Cytometers, Ann Arbor,  Samples were monitored using FACS (Accuri C6, BD Biosciences). The term After 24 h, protein lysates were prepared as mentioned in the manual. 500 μg of  The BD Accuri C6 Flow Cytometer System . The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance.

Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Fixed optics and capillary sheath-flow fluidics make the BD Accuri C6 Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Turn on the computer and log in with your UCF info.
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BD Accuri C6 Flow Cytometer Instrument Manual Refer to the BD Accuri C6 Safety and Limitations Manual (PN 7820091) for complete safety information. When setting up the cytometer, note the details in the following sections to maximize the instrument’s performance. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Manuals and User Guides for BD Accuri C6. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual BD Accuri C6 Instrument Manual (31 pages) NOTE: This update does not impact the BD Accuri™ C6 Plus. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016.

It is small enough to Bd Accuri C6 User Manual. Articles Introducing the BD Accuri™ C6 Plus. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. There are two options here: Buy from Accuri, or make your own citric acid solution.
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BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. Page 19: Decontamination Fluid Cycle 6.

If you have questions or require further clarification, see the BD Accuri C6 Flow Cytometer Instrument Manual or contact Accuri Technical Support.